![]() ![]() We also use viSNE and mass cytometry to compare leukemia diagnosis and relapse samples, and to identify a rare leukemia population reminiscent of minimal residual disease. Healthy bone marrow automatically maps into a consistent shape, whereas leukemia samples map into malformed shapes that are distinct from healthy bone marrow and from each other. We integrated mass cytometry with viSNE to map healthy and cancerous bone marrow samples. viSNE plots individual cells in a visual similar to a scatter plot, while using all pairwise distances in high dimension to determine each cell's location in the plot. Here we present viSNE, a tool that allows one to map high-dimensional cytometry data onto two dimensions, yet conserve the high-dimensional structure of the data. However, because these technologies can measure dozens of parameters simultaneously in individual cells, data interpretation can be challenging. New high-dimensional, single-cell technologies offer unprecedented resolution in the analysis of heterogeneous tissues. ![]()
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